pcmv:ABI5, the full-length coding region of ABI4 and ABI5 were ampliﬁed by PCR and then inserted into the pcmv-3xHA plasmid. To generate the DGAT1-luciferase reporter vector, the DGAT1 pro- moter sequence (1000 to + 226 bp) was ampliﬁed by PCR and in-

Nodes: Network nodes represent proteins

To further investigate the relationship between ABI5 and ABI5 acts as a positive regulator in ABA signaling, regulating both seed germination and post‐germinative seedling development (Finkelstein and Lynch 2000). ABA not only activates ABI5 transcription, but also promotes ABI5 accumulation (Finkelstein and Lynch 2000; Lopez‐Molina et al. 2001). Reduced transcription of FLC results, at least partially, from the abnormal splicing and reduced transcript abundance of ABSCISIC ACID‐INSENSITIVE 5 (ABI5), which encodes an activator of FLC in ABA‐regulated flowering signaling. Additionally, the expression of AtU2AF65b is promoted by ABA. The gene can be activated by the basic leucine zipper transcription factor ABI5. Expressed predominantly in provascular tissues with the strongest expression in the root tip; Belongs to the small hydrophilic plant seed protein family (152 aa) ABI5 is a central regulator of seed germination and post-germinative growth in Arabidopsis (Lopez-Molina et al., 2001).

Nodes: Network nodes represent proteins To elucidate the role of ABI5 in ped3‐3, we produced a ped3‐3 abi5 double mutant by crossing ped3‐3 with an abi5 mutant in the Landsberg erecta background. This mutant had a deletion at the ABI5 locus, which includes four genes: AT2g36260, AT2g36261, ABI5 (At2g36270) and AT2g36280 (Figure S1). Nodes: Network nodes represent proteins 2020-09-07 · ABI5 is a central regulator of seed germination and post-germinative growth in Arabidopsis (Lopez-Molina et al., 2001). To test whether ABT affects early developmental processes through ABI5, we performed western blot analysis to assess ABI5 protein levels in imbibed abt-1D, abt-2, and WT seeds treated with ABA TAIR: AT1G45249: UniProt: Q9M7Q4: Position: 1. AA seq: 416 aa AA seq DB search AT2G36270 (ABI5) 09180 Brite Hierarchies 09182 Protein families: genetic RNA splicing is a fundamental mechanism contributing to the definition of the cellular protein population in any given environmental condition.

Together with EDR1, may regulate endocytic trafficking and/or the formation of signaling complexes on trans-Golgi network (TGN)/ early endosome (EE) vesicles during stress responses ABI5 is a transcription factor that participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth [[cite:PUB00019236], [cite:PUB00071025]]. It is also involved in the sugar signalling response in plants [[cite:PUB00071026]].
Acts as a negative regulator of abscisic acid (ABA) response during germination through the ubiquitin-mediated proteolysis of ABI5/DPBF1. Övergång mellan gibberellinsyra (GA) och abscisinsyra (ABA) reglerar frögroning.

ABI5 is a key component in ABA-triggered pathways during germination, seedling establishment, and vegetative growth (Lopez-Molina et al., 2001); in addition, it has been reported to play certain roles in nitrogen assimilation and signaling (Signora et al., 2001).

The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. The dgat1 mutant (CS3861) was purchased from TAIR. An abi4‐1 mutant line in a Col‐0 background and an abi5‐1 mutant line in a Ws background were obtained from Dr. Dapeng Zhang. We generated the double mutant (abi4abi5) by crossing the single mutants.

ABI5 ABA insensitive 5 RT-PCR Reverse-transcriptase polymerase chain reaction LEA Late embryogenesis abundant genes WT Wild type Introduction Rice that provides staple food for half of the world popula-tion relies heavily on water availability for cultivation. As the shortage of fresh water for agricultural irrigation wors-

All these physiological, biochemical, and molecular evidence support the idea that HDA6 functions together with DET1 to coordinately inhibit the transcriptional activity of FHY3. The dgat1 mutant (CS3861) was purchased from TAIR. An abi4‐1 mutant line in a Col‐0 background and an abi5‐1 mutant line in a Ws background were obtained from Dr. Dapeng Zhang.

abi5 Encodes a member of the basic leucine zipper transcription factor family, involved in ABA signalling during seed maturation and germination. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. The functional relationship between AtU2AF65b and ABI5 was further tested by using the abi5 atu2af65b‐1 double mutant.
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Can also trans-activate its own promoter, suggesting that it is autoregulated. 2016-09-14 The functional relationship between AtU2AF65b and ABI5 was further tested by using the abi5 atu2af65b‐1 double mutant.

Background ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters.
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ABI5 acts as a positive regulator in ABA signaling, regulating both seed germination and post‐germinative seedling development (Finkelstein and Lynch 2000). ABA not only activates ABI5 transcription, but also promotes ABI5 accumulation (Finkelstein and Lynch 2000; Lopez‐Molina et al. 2001).

Download Curated Data for this ABI5 is induced by low-nitrogen stress, but the function of ABI5 with respect to TAG accumulation under stress is still unknown. To further investigate the relationship between ABI5 and the stress response, ABI5 expression was observed in 7-d-old seedlings cultivated in the presence of different stressors. pcmv:ABI5, the full-length coding region of ABI4 and ABI5 were ampliﬁed by PCR and then inserted into the pcmv-3xHA plasmid.